Browsing Waterloo Institute for Nanotechnology by Issue Date
Now showing items 21-40 of 193
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Metal-Induced Specific and Nonspecific Oligonucleotide Folding Studied by FRET and Related Biophysical and Bioanalytical Implications
(Wiley, 2012-01-13)Metal induced nucleic acid folding has been extensively studied with ribozymes, DNAzymes, tRNA and riboswitches. These RNA/DNA molecules usually have a high content of double-stranded regions to support a rigid scaffold. ... -
Droplet formation in microfluidic T-junction generators operating in the transitional regime. II. Modeling
(American Physical Society Physics, 2012-01-26)This is the second part of a two-part study on the generation of droplets at a microfluidic T-junction operating in the transition regime. In the preceding paper [Phys. Rev. E 85, 016322 (2012)], we presented our experimental ... -
Droplet formation in microfluidic T-junction generators operating in the transitional regime. I. Experimental observations
(American Physical Society Physics, 2012-01-26)This is the first part of a two-part study on the generation of droplets at a microfluidic T-junction operating in the transition regime where confinement of the droplet creates a large squeezing pressure that influences ... -
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
(Wiley, 2012-02-10)Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported. -
Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles
(American Chemical Society, 2012-02-28)Single-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental ... -
Hydrogel porosity controlling DNA-directed immobilization of gold nanoparticles revealed by DNA melting and scanning helium ion microscopy
(Royal Society of Chemistry, 2012-04-07)Immobilization of nanomaterials is important for many applications, including sensor development, biomaterials design and catalysis. DNA-directed immobilization has been widely used because of its high specificity and ... -
Biomimetic sensing based on chemically induced assembly of a signaling DNA aptamer on a fluid bilayer membrane
(Royal Society of Chemistry, 2012-04-18)The adenosine aptamer was split into two halves and linked to a fluid liposome surface; addition of adenosine resulted in aptamer assembly, which did not occur if the split aptamer was attached to silica nanoparticles, ... -
Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine
(American Chemical Society, 2012-04-25)With a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding ... -
Molecular Beacon Lighting up on Graphene Oxide
(American Chemical Society, 2012-05-01)A molecular beacon (MB) is comprised of a fluorophore and a quencher linked by a DNA hairpin. MBs have been widely used for homogeneous DNA detection. In addition to molecular quenchers, many nanomaterials such as graphene ... -
Instantaneous and Quantitative Functionalization of Gold Nanoparticles with Thiolated DNA Using a pH-Assisted and Surfactant-Free Route
(American Chemical Society, 2012-05-02)The attachment of thiolated DNA to gold nanoparticles (AuNPs) has enabled many landmark works in nanobiotechnology. This conjugate chemistry is typically performed using a salt-aging protocol where, in the presence of an ... -
Ultrahigh Nanoparticle Stability against Salt, pH, and Solvent with Retained Surface Accessibility via Depletion Stabilization
(American Chemical Society, 2012-06-20)For many applications, it is desirable to stabilize colloids over a wide range of buffer conditions while still retaining surface accessibility for adsorption and reaction. Commonly used charge or steric stabilization ... -
Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications
(Royal Society of Chemistry, 2012-06-28)The interaction between DNA and inorganic surfaces has attracted intense research interest, as a detailed understanding of adsorption and desorption is required for DNA microarray optimization, biosensor development, and ... -
Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH
(Royal Society of Chemistry, 2012-07-11)Blue fluorescent gold nanoclusters were prepared in the presence of poly-cytosine DNAs at low pH and poly-adenine at neutral pH using citrate as the reducing agent; various buffer conditions affecting the synthesis have ... -
Dissecting the effect of anions on Hg2+ detection using a FRET based DNA probe
(Royal Society of Chemistry, 2012-08-07)Many biosensors have been developed to detect Hg2+ using thymine-rich DNA. While sensor response to various cations is often studied to demonstrate selectivity, the effect of anions has been largely overlooked. Anions may ... -
Droplet formation in microfluidic T-junction generators operating in the transitional regime. III. Dynamic surfactant effects
(American Physical Society Physics, 2012-08-13)This study extends our previous work on droplet generation in microfluidic T-junction generators to include dynamic interfacial tension effects created by the presence of surfactants. In Paper I [T. Glawdel, C. Elbuken, ... -
Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide
(American Chemical Society, 2012-10-09)Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular ... -
Fast pH-assisted functionalization of silver nanoparticles with monothiolated DNA
(Royal Society of Chemistry, 2012-10-18)Attaching monothiolated DNA to silver nanoparticles has been achieved at pH 3.0 in 30 minutes and difficulties associated with DNA attachment to AgNPs at neutral pH have been revealed by studying DNA adsorption kinetics. -
Exploring the thermal stability of DNA-linked gold nanoparticles in ionic liquids and molecular solvents
(Royal Society of Chemistry, 2012-11-01)While water is the most commonly used solvent for DNA, many co-solvents have been added for various applications. Ionic liquids (ILs) are molten salts at around room temperature. ILs have been tested as a green solvent for ... -
DNA-Functionalized Gold Nanoparticles in Macromolecularly Crowded Polymer Solutions
(American Chemical Society, 2012-11-15)DNA-functionalized gold nanoparticles (AuNPs) are one of the most commonly used reagents in nanobiotechnology. They are important not only for practical applications in analytical chemistry and drug delivery, but also for ... -
Instantaneous Attachment of an Ultrahigh Density of Nonthiolated DNA to Gold Nanoparticles and Its Applications
(American Chemical Society, 2012-12-11)The last 16 years have witnessed the landmark development of polyvalent thiolated DNA-functionalized gold nanoparticles (AuNP's) possessing striking properties within the emerging field of nanobiotechnology. Many novel ...