Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET
| dc.contributor.author | Muraih, Jawad K. | |
| dc.contributor.author | Palmer, Michael | |
| dc.date.accessioned | 2017-04-13 13:32:09 (GMT) | |
| dc.date.available | 2017-04-13 13:32:09 (GMT) | |
| dc.date.issued | 2012-07 | |
| dc.identifier.uri | https://doi.org/10.1016/j.bbamem.2012.02.019 | |
| dc.identifier.uri | http://hdl.handle.net/10012/11658 | |
| dc.description | The final publication is available at Elsevier via http://doi.org/10.1016/j.bbamem.2012.02.019 © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
| dc.description.abstract | Daptomycin is a lipopeptide antibiotic that kills Gram-positive bacteria by depolarizing their cell membranes. This antibacterial action of daptomycin is correlated with the formation of membrane-associated oligomers. We here examine the number of subunits contained in one oligomer using fluorescence resonance energy transfer (FRET). The results suggest that the oligomer contains approximately 6 to 7 subunits, or possibly twice this number if it spans both membrane monolayers. | en |
| dc.description.sponsorship | This work was supported by an NSERC operating grant to M. Palmer. J. Muraih was supported by a scholarship from the government of Iraq. | en |
| dc.language.iso | en | en |
| dc.publisher | Elsevier | en |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | Antibiotic daptomycin | en |
| dc.subject | Bactericidal activity | en |
| dc.subject | Binding | en |
| dc.subject | Daptomycin | en |
| dc.subject | Energy-transfer | en |
| dc.subject | Fluorescence resonance energy transfer | en |
| dc.subject | Lipopeptide antibiotics | en |
| dc.subject | Oligomer stoichiometry | en |
| dc.subject | Staphylococcus-aureus | en |
| dc.title | Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET | en |
| dc.type | Article | en |
| dcterms.bibliographicCitation | Muraih, J. K., & Palmer, M. (2012). Estimation of the subunit stoichiometry of the membrane-associated daptomycin oligomer by FRET. Biochimica Et Biophysica Acta-Biomembranes, 1818(7), 1642–1647. https://doi.org/10.1016/j.bbamem.2012.02.019 | en |
| uws.contributor.affiliation1 | Faculty of Science | en |
| uws.contributor.affiliation2 | Chemistry | en |
| uws.typeOfResource | Text | en |
| uws.peerReviewStatus | Reviewed | en |
| uws.scholarLevel | Faculty | en |
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