dc.contributor.author | Zhou, Wenhu | |
dc.contributor.author | Ding, Jinsong | |
dc.contributor.author | Liu, Juewen | |
dc.date.accessioned | 2017-04-28 16:11:55 (GMT) | |
dc.date.available | 2017-04-28 16:11:55 (GMT) | |
dc.date.issued | 2016-05-17 | |
dc.identifier.uri | http://dx.doi.org/10.1002/cbic.201500690 | |
dc.identifier.uri | http://hdl.handle.net/10012/11786 | |
dc.description | This is the peer reviewed version of the following article: Zhou, W., Ding, J., & Liu, J. (2016). An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 ’-5 ’-Linked RNA. Chembiochem, 17(10), 890–894, which has been published in final form at https://doi.org/10.1002/cbic.201500690. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en |
dc.description.abstract | RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that specifically cleaves 2'-5' RNA by using Ce3+ as the metal cofactor, but leaves the 3'-5' counterpart intact. This Ce5 DNAzyme requires trivalent light lanthanide ions and shows a rate of 0.16 min(-1) in the presence of 10 mm Ce3+; the activity decreases with heavier lanthanide ions. This is the fastest DNAzyme reported for this reaction, and it might enable applications in chemical biology. As a proof-of-concept, using this DNAzyme, the reactions between phosphorothioate-modified RNA and strongly thiophilic metals (Hg2+ and Tl3+) were studied as a function of pH. | en |
dc.description.sponsorship | University of Waterloo; Natural Sciences and Engineering Research Council of Canada (NSERC); Foundation for Shenghua Scholar of Central South University; National Natural Science Foundation of China [21301195]; China Scholarship Council (CSC) [201406370116] | en |
dc.language.iso | en | en |
dc.publisher | Wiley | en |
dc.subject | DNA Enzymes | en |
dc.subject | Metal | en |
dc.subject | Deoxyribozymes | en |
dc.subject | Selectivity | en |
dc.subject | Catalysis | en |
dc.subject | Cofactors | en |
dc.subject | Ligase | en |
dc.subject | Beacon | en |
dc.subject | Desulfurization | en |
dc.subject | Linkages | en |
dc.title | An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 '-5 '-Linked RNA | en |
dc.type | Article | en |
dcterms.bibliographicCitation | Zhou, W., Ding, J., & Liu, J. (2016). An Efficient Lanthanide-Dependent DNAzyme Cleaving 2 ’-5 ’-Linked RNA. Chembiochem, 17(10), 890–894. https://doi.org/10.1002/cbic.201500690 | en |
uws.contributor.affiliation1 | Faculty of Science | en |
uws.contributor.affiliation2 | Chemistry | en |
uws.contributor.affiliation3 | Waterloo Institute for Nanotechnology (WIN) | en |
uws.typeOfResource | Text | en |
uws.peerReviewStatus | Reviewed | en |
uws.scholarLevel | Faculty | en |