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dc.contributor.authorDubinski, Alicia F.
dc.contributor.authorCamasta, Raffaele
dc.contributor.authorSoule, Tyler G. B.
dc.contributor.authorReed, Bruce H.
dc.contributor.authorGlerum, D. Moira 13:37:11 (GMT) 13:37:11 (GMT)
dc.descriptionThe final publication is available at Elsevier via © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
dc.description.abstractThe assembly of cytochrome c oxidase (COX) is essential for a functional mitochondrial respiratory chain, although the consequences of a loss of assembled COX at yeast stationary phase, an excellent model for terminally differentiated cells in humans, remain largely unexamined. In this study, we show that a wild-type respiratory competent yeast strain at stationary phase is characterized by a decreased oxidative capacity, as seen by a reduction in the amount of assembled COX and by a decrease in protein levels of several COX assembly factors. In contrast, loss of assembled COX results in the decreased abundance of many mitochondrial proteins at stationary phase, which is likely due to decreased membrane potential and changes in mitophagy. In addition to an altered mitochondrial proteome, COX assembly mutants display unexpected changes in markers of cellular oxidative stress at stationary phase. Our results suggest that mitochondria may not be a major source of reactive oxygen species at stationary phase in cells lacking an intact respiratory chain.en
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN-227415-2012)en
dc.description.sponsorshipUndergraduate Research Internship (URI) Program at the University of Waterlooen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectCytochrome oxidase (complex IV) assemblyen
dc.subjectOxidative stressen
dc.subjectStationary phaseen
dc.titleConsequences of cytochrome c oxidase assembly defects for the yeast stationary phaseen
dcterms.bibliographicCitationDubinski, A. F., Camasta, R., Soule, T. G. B., Reed, B. H., & Glerum, D. M. (2018). Consequences of cytochrome c oxidase assembly defects for the yeast stationary phase. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(6), 445–458.
uws.contributor.affiliation1Faculty of Scienceen
uws.contributor.affiliation3Waterloo Institute for Nanotechnology (WIN)en

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