Ligand Specificity and Affinity in the Sulforhodamine B Binding RNA Aptamer
Abstract
Binding affinity and selectivity are critical properties of aptamers that must be optimized for any
application. The sulforhodamine B binding RNA aptamer (SRB‐2) is a somewhat promiscuous aptamer
that can bind ligands that vary markedly in shape, size and charge. Here we categorize potential ligands
based on their binding mode and structural characteristics required for high affinity and selectivity.
Several known and potential ligands of SRB‐2 were screened for binding affinity using LSPR, ITC and
NMR spectroscopy. The study shows that rhodamine B has the ideal structural and electrostatic
properties for selective and high‐affinity binding of the SRB‐2 aptamer.
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Cite this version of the work
Kyle Piccolo, Brooke McNeil, Jeff Crouse, Su Ji Lim, W. Scott Hopkins, Thorsten Dieckmann
(2020).
Ligand Specificity and Affinity in the Sulforhodamine B Binding RNA Aptamer. UWSpace.
http://hdl.handle.net/10012/17658
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