Browsing Theses by Supervisor "Aucoin, Marc"
Now showing items 1-18 of 18
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The construction and characterization of a synthetic SARS VLP comprised of betacoronavirus consensus sequence-encoded viral components
(University of Waterloo, 2022-12-22)In 2019, a novel coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged from Wuhan, China leading to the COVID-19 pandemic. Although there was an outbreak of severe acute respiratory syndrome ... -
Design of a DNA-Encoded Human Papilloma Virus-Like Particle Displaying a Vascular Endothelial Growth Factor Antagonistic Peptide for Characterization in Mammalian Cells
(University of Waterloo, 2022-12-21)Cancer immunotherapy has evolved as an effective platform for the treatment of a variety of cancer types by enhancing and/or modulating the functionality of the immune system to target cancer cells and consequently mitigate ... -
Development of a Serum-Free Chemically Defined Medium for Adherent and Suspension Culture
(University of Waterloo, 2022-01-07)The development of suspension cell lines is a sought after holy grail for bioprocess development. Suspension cell lines allow for easier scale up and better volumetric productivity for most biotherapeutics, including vaccine ... -
Droplet Production of Biological non-Newtonian Fluids and Bioassay Applications in a Microfluidic Network
(University of Waterloo, 2020-08-31)Droplet-based microfluidics has been developed over the last two decades and presented as a promising platform for biochemical assay. This promise relies on the system’s ability to quantify the reagents accurately with ... -
Establishing the Virucidal Properties of a Copper-Nickel-Zinc Alloy using a Genetically Modified Insect Virus
(University of Waterloo, 2019-08-28)Healthcare associated infections (HAIs) cause a significant financial burden on the healthcare industry. To reduce incidence of HAIs, strategies for cleaning and infection control have been developed to target common ... -
Examination of binding elements and conditions of Cryptosporidium parvum oocysts to assess its detection potential in water
(University of Waterloo, 2022-09-29)Cryptosporidium parvum is an intestinal parasite that can be spread through environmental and recreational waters, most often in the form of oocysts. As an oocyst, this parasite is resistant to chlorine disinfection, and ... -
From Metabolite Concentration to Flux – A Systematic Assessment of Error in Cell Culture Metabolomics
(University of Waterloo, 2016-08-05)The growing availability of genomic, transcriptomic, and metabolomic data has opened the door to the synthesis of multiple levels of information in biological research. As a consequence, there has been a push to analyze ... -
Generation and Characterization of Dual-Fluorescent Influenza Virus-like Particles (VLPs) in Insect Cells
(University of Waterloo, 2019-01-04)The Baculovirus Expression Vector System (BEVS) has been widely used to produce recombinant proteins, especially for complex biopharmaceuticals. In recent years, the insect-baculovirus system has proved its value as a ... -
An In-Depth Look at Repeated Propagation of Multiple Viruses Simultaneously in Insect Cell Culture
(University of Waterloo, 2020-01-22)It is well known that the insect cell-baculovirus expression vector system (IC-BEVS) can be used for the production of various proteins of interest and virus-like particles (VLPs). In the past, many higher order proteins ... -
Investigation of excipients for the stabilization of HSV-2 vaccine candidate ACAM529
(University of Waterloo, 2018-05-01)Herpes simplex virus type 2 (HSV-2) is a highly infectious pathogen that causes genital ulcerative disease and it affects millions of people worldwide. ACAM529 is a promising new live replication-deficient vaccine candidate ... -
Partial Characterization of Yeast Hydrolysates for Insights on Chemically Defined Media for Sf-9 Insect Cells
(University of Waterloo, 2017-10-18)Yeast extract, or other varieties of yeast hydrolysate, have been used in combination with a lipid supplement to completely eliminate the need for fetal bovine serum during in vitro cultivations of insect cells. This has ... -
Supplementation Strategies for Tuning Glycosylation of Monoclonal Antibodies and Enhancing Growth in Mammalian Cell Culture by Omics Analysis
(University of Waterloo, 2018-04-17)Two fundamental objectives of bioprocess engineering are to increase productivity and improve product quality. Glycosylation is a critical and variable factor in the quality of several therapeutic proteins, particularly ... -
Toward Optimization of the Baculovirus Expression Vector System - Development of Genetic Tools to Improve Biologics Production
(University of Waterloo, 2021-08-25)Small-molecule drugs have dominated the pharmaceutical industry and physician's prescription pads since the beginning of modern medicine. The most recent decades, however, are most aptly characterized as the era of ... -
Towards a Chemically Defined Medium for Sf-9 Cell Culture: Micronutrients Reduce Dependence on Yeast Extract
(University of Waterloo, 2020-01-08)Spodoptera frugiperda clonal isolate 9 (Sf-9) insect cells in conjunction with recombinant baculovirus are an industrially relevant system for producing biologics. Sf-9 cells are capable of robust high-density growth in ... -
Towards the Development of a ϕC31Int-Competent Sf9 Cell Line
(University of Waterloo, 2018-12-05)The Sf9 cell line, as a component of the Baculovirus Expression Vector System (BEVS), is a popular platform for industrial-scale production of protein products. However, the natural course of the BEVS results in host-cell ... -
Tracking Uptake, Assembly and Egress of ACAM529 Herpes Simplex Virus Type 2 in Complementary Vero Cells for Enhanced Virus Recovery
(University of Waterloo, 2016-01-06)Herpes simplex virus (HSV) type 2 is the cause of one of the most wide-spread human infections in the world, affecting millions of people and circumventing all past attempts to cure or prevent infection. One of the ... -
Understanding the Sf9 – Baculovirus Expression Vector System: Exploring the Metabolic Basis of the Cell Density Effect through Nuclear Magnetic Resonance Spectroscopy and Flow Cytometry Analysis of Fluorescent Protein Accumulation
(University of Waterloo, 2020-05-15)It is well established that the baculovirus expression vector system (BEVS) can serve as an important, effective production system for various biologicals. Knowing that the cells can serve as a production platform is ... -
Use and Control of Co-Expression in the Baculovirus-Insect Cell System for the Production of Multiple Proteins and Complex Biologics
(University of Waterloo, 2016-08-19)The ease of use and versatility of the Baculovirus Expression Vector System (BEVS) has made it one of the most widely used platforms for recombinant protein production. In the last ten years, the system has even gained ...