Browsing Science (Faculty of) by Title
Now showing items 351-370 of 3045
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Characterization of Mrc1 during Replication Initiation in Saccharomyces cerevisiae
(University of Waterloo, 2022-01-12)DNA replication is a highly specific process that is completed under the control of several factors, to ensure faithful duplication of the genome. Mrc1 (Claspin in mammalian cells) is a protein that is a part of a fork ... -
Characterization of novel FHA domain ligand binding motifs
(University of Waterloo, 2016-01-25)Rad53, a key checkpoint protein in Saccharomyces cerevisiae, has a central catalytic kinase domain as well as two forkhead-associated (FHA) domains, FHA1 and FHA2. Along with the extensively studied phosphothreonine binding ... -
Characterization of Novel Protease Flagellinolysin in Pseudoalteromonas tunicata
(University of Waterloo, 2024-01-03)Flagellinolysins are a family of novel enzymes that act both as structural flagellins and proteases. Although previously identified and shown to be both structurally integrated into filaments and proteolytically active, ... -
Characterization of Orc6 function following pre-replicative complex assembly in Saccharomyces cerevisiae
(University of Waterloo, 2008-10-22)Pre-replicative complex (pre-RC) components the origin recognition complex (ORC), Cdc6, and Cdt1, play key roles in the recruitment, and loading of the replicative helicase, the minichromosome maintenance complex (Mcm2-7), ... -
Characterization of p53 in rainbow trout cell lines
(University of Waterloo, 2010-09-27)The tumour suppressor protein p53 is a critical protein in the DNA damage checkpoint pathway. It acts as a transcription factor that is involved in initiating the apoptotic pathway; disruption of this pathway can lead to ... -
Characterization of PIBSI Dispersants and Their PIBSA Components by Pyrene Excimer Fluorescence
(University of Waterloo, 2020-12-18)Methods are introduced to characterize the products resulting from the coupling of a polyisobutylene terminated at one end with a succinic anhydride (PIBSA) and a polyamine to generate PIBSI products where the polyamine ... -
Characterization of Polysaccharides in Starch using Fluorescence Techniques
(University of Waterloo, 2020-09-25)Amylose and amylopectin, which are the two main constituents of starch, were characterized using the pyrene excimer fluorescence (PEF) technique. First, an amylose sample was randomly labeled with the dye pyrene to yield ... -
Characterization of Self-Assembling Quinoline- Based Foldamers by Fluorescence Anisotropy
(University of Waterloo, 2019-09-24)Foldamers represent a family of synthetic macromolecules which, like their biological counterparts, are able to adopt a well-defined conformation in solution. Oligoquinoline-carboxamides (Qn) are a group of foldamers that ... -
Characterization of Several Small Biologically Relevant Molecules by Infrared Multiple Photon Dissociation Spectroscopy and Electronic Structure Calculations
(University of Waterloo, 2011-04-29)Infrared multiple photon dissociation (IRMPD) spectroscopy has been coupled with electronic structure calculations in order to elucidate the structures of several small biological molecules including: uracil, 5-fluorouracil, ... -
Characterization of Starch Nanoparticles by Fluorescence Techniques
(University of Waterloo, 2014-05-21)Abstract The properties of starch nanoparticles (SNPs) labeled with the fluorescent dye pyrene (Py-SNPs) were probed by using fluorescence quenching, pyrene excimer formation, and transmission electron microscopy (TEM). ... -
Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae.
(University of Waterloo, 2012-01-03)Initiation of DNA replication requires the action of the Dbf4/Cdc7 kinase complex (DDK) which is also a phosphorylation target of Rad53 kinase in the S-phase checkpoint. DDK is thought to trigger DNA replication by ... -
Characterization of the Behaviour of Solution-Responsive Polymers by Fluorescence
(University of Waterloo, 2014-04-17)Two families of amphiphilic polymers were characterized with respect to their ability to respond to changing solution conditions using steady-state and time-resolved fluorescence. Firstly, a series of 5 sequential amphiphilic ... -
Characterization of the chloroplast protein import receptors in the single-cell C4 species Bienertia sinuspersici
(University of Waterloo, 2012-04-25)Bienertia sinuspersici is one of the three currently known land plants which perform C4 photosynthesis without the conventional dual-cell system, but rather through intracellular compartmentation of organelles and enzymes ... -
Characterization of the Conformation and Internal Backbone Dynamics of a-Polyglucans by Pyrene Excimer Fluorescence
(University of Waterloo, 2021-11-23)The conformation and internal backbone dynamics (IBD) of several pyrene-labeled polysaccharides (Py-PSs), namely Py-Amylopectin, Py-Glycogen, Py-Dextran, and Py-Pullulan, were successfully examined with a combination of ... -
A characterization of the controls of the nitrogen and oxygen isotope ratios of biologically-produced nitrous oxide and nitrate in soils
(University of Waterloo, 2011-08-02)Nitrous oxide (N₂O) is a potent greenhouse gas, an important driver of climate change, and its concentration in the atmosphere is rising at an unprecedented rate. Agriculture is the leading contributor of all the anthropogenic ... -
Characterization of the Distribution of Pyrene Molecules in Confined Geometries with the Model Free Analysis
(American Chemical Society, 2017-12-21)Evidence is provided showing that global Model Free Analysis (MFA) of monomer and excimer fluorescence decays of pyrene dissolved in aqueous solutions of sodium dodecyl sulfate (SDS) provides the same structural and dynamic ... -
Characterization of the Downstream Effects of the Rainbow Trout (Oncorhynchus mykiss) Chemokine, CK-2 on Macrophages
(University of Waterloo, 2010-05-25)Chemokines are chemotactic cytokines that have the ability to attract leukocytes and guide them via a concentration gradient to sites of injury/infection. These small, basic proteins are secreted when induced and act on ... -
Characterization of the expression and function of <em>Rana catesbeiana</em> HSP30 and <em>Xenopus laevis</em> HSP27
(University of Waterloo, 2006)Exposure of cells to environmental or chemical stressors will initiate the heat shock response, which is mediated by heat shock proteins. Heat shock proteins are molecular chaperones which are classified by size into six ... -
Characterization of the FHA domain involved in Saccharomyces cerevisiae dNTP regulation.
(University of Waterloo, 2018-04-26)Over 2000 Forkhead-associated (FHA) domain-containing proteins exhibiting diverse functions, such as kinases, phosphatases, and transcription factors, have been identified to date in both eukaryotic and prokaryotic organisms. ... -
Characterization of the Interactions Between DNA and Oppositely Charged Species by Fluorescence
(University of Waterloo, 2011-01-24)The characterization of the interactions between DNA and oppositely charged species were characterized by fluorescence. Quantitative and insightful information on the distribution of positively charged species around the ...