Browsing Science (Faculty of) by Title
Now showing items 768-787 of 3047
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Diversity of Viral Communities in a Northern Temperate Lake through Metagenomics
(University of Waterloo, 2023-04-05)Viruses in oceanic environments play pivotal roles in regulating nutrient transfer and increasing rates of carbon transport. They have also been implicated in the regulation of micro-organism community dynamics through ... -
DNA Adsorption by Indium Tin Oxide (ITO) Nanoparticles
(American Chemical Society, 2015-01-13)The high conductivity and optical transparency of indium tin oxide (ITO) has made it a popular material in the electronic industry. Recently, its application in biosensors is also explored. To understand its biointerface ... -
DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection
(Royal Society of Chemistry, 2014-05-01)Iron oxide nanoparticles adsorb fluorescently labeled DNA oligonucleotides via the backbone phosphate and quench fluorescence. Arsenate displaces adsorbed DNA to increase fluorescence, allowing detection of arsenate down ... -
DNA Adsorption by ZnO Nanoparticles near Its Solubility Limit: Implications for DNA Fluorescence Quenching and DNAzyme Activity Assays
(American Chemical Society, 2016-06-07)Zinc oxide (ZnO) is a highly important material, and Zn2+ is a key metal ion in biology. ZnO and Zn2+ interconvert via dissolution and hydrolysis/condensation. In this work, we explore their interactions with DNA, which ... -
DNA Adsorption, Desorption, and Fluorescence Quenching by Graphene Oxide and Related Analytical Application
(University of Waterloo, 2011-09-20)Graphene is a single layer of graphite with many unique mechanical, electrical, and optical properties. In addition, graphene is also known to adsorb wide range of biomolecules including single-stranded DNA. On the other ... -
DNA and Hydrogels for Sensing and Binding Purine Nucleosides
(University of Waterloo, 2021-08-27)Purine nucleosides, such as adenosine and guanosine, are important biomolecules to regulate physiological functions, including maintain heart and brain health, exert inflammatory responses, and take part in metabolisms. ... -
DNA and Metal Ion Mediated Modification of Nanomaterials
(University of Waterloo, 2021-09-01)DNA-modified nanomaterials have been applied in diverse areas such as biosensing, catalysis, drug delivery, and biomedical diagnostics. Metal ion mediated DNA conjugation is an important strategy for the construction of ... -
DNA duplex stabilization in crowded polyanion solutions
(Royal Society of Chemistry, 2013-02-14)The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect. -
DNA functionalized soft materials: preparation, biophysical properties and analytical applications.
(University of Waterloo, 2013-01-02)Bio-nanotechnology is the use of biomolecules to control both the structure and property of nanomaterials. No biomolecule has been employed more often than DNA as exemplified in the numerous demonstrations of DNA-directed ... -
DNA in Ionic Liquids and Polyelectrolytes
(University of Waterloo, 2013-08-30)DNA has been widely studied in a variety of solvents. The majority of these solvents consist of either aqueous or organic components. The presence of ions or salts in these solvents can further alter DNA properties by ... -
DNA stabilized fluorescent metal nanoclusters for biosensor development
(Elsevier, 2014-03-22)Fluorescent silver, gold and copper nanoclusters (NCs) have emerged for biosensor development. Compared to semiconductor quantum dots, there is less concern about the toxicity of metal NCs, which can be more easily conjugated ... -
DNA stabilized silver nanoclusters for ratiometric and visual detection of Hg2+ and its immobilization in hydrogels
(Elsevier, 2013-04-11)DNA oligomers are particularly interesting templates for making silver nanoclusters (AgNCs) as different emission colors can be obtained by varying the DNA sequence. Many AgNCs have been used as Hg2+ sensors since Hg2+ ... -
DNA-Functionalized Gold Nanoparticles in Macromolecularly Crowded Polymer Solutions
(American Chemical Society, 2012-11-15)DNA-functionalized gold nanoparticles (AuNPs) are one of the most commonly used reagents in nanobiotechnology. They are important not only for practical applications in analytical chemistry and drug delivery, but also for ... -
DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection
(American Chemical Society, 2010-12-22)Highly sensitive and selective DNA detection plays a central role in many fields of research, and various assay platforms have been developed. Compared to homogeneous DNA detection, surface-immobilized probes allow washing ... -
DNA-Length-Dependent Fluorescence Signaling on Graphene Oxide Surface
(Wiley, 2012-02-10)Fluorescence energy transfer to graphene oxide is studied using covalently linked DNA probes ranging from 4 to 70 base pairs. The characteristic distance and mechanism of energy transfer are reported. -
DNA/Metal Oxide Nanoconjugates: Fundamental Understandings and Analytical Applications
(University of Waterloo, 2016-07-29)DNA-functionalized nanomaterials have shown versatile applications in biosensor development, biomedical diagnostics, therapy, and catalysis. DNA is attractive for this purpose for its programmable structure, molecular ... -
DNAzyme Hybridization, Cleavage, Degradation and Sensing in Undiluted Human Blood Serum
(American Chemical Society, 2015-04-07)RNA-cleaving DNAzymes provide a unique platform for developing biosensors. However, a majority of the work has been performed in clean buffer solutions, while the activity of some important DNAzymes in biological sample ... -
A DNAzyme requiring two different metal ions at two distinct sites
(Oxford University Press, 2015-12-10)Most previously reported RNA-cleaving DNAzymes require only a single divalent metal ion for catalysis. We recently reported a general trivalent lanthanide-dependent DNAzyme named Ce13d. This work shows that Ce13d requires ... -
Does intersex matter? A case study of rainbow darter in the Grand River
(University of Waterloo, 2016-12-21)Endocrine disrupting compounds (EDCs) are present in the environment and can have negative effects on the health of wildlife. Aquatic organisms residing near the outfalls of municipal wastewater effluent (MWWE) are chronically ... -
Domain II (S5-P) region in Lymnaea T-type calcium channels and its role in determining biophysical properties, ion selectivity and drug sensitivity
(University of Waterloo, 2014-05-27)Invertebrate T-type calcium channels cloned from the great pond snail, Lymnaea Stagnalis (LCav3) possess highly sodium permeant ion channel currents by means of alternative splicing of exon 12. Exon 12 is located on the ...