Browsing Biology by Title
Now showing items 81-100 of 599
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Characterization of Genes involved In Development and Senescence
(University of Waterloo, 2006)Plant development is complex and highly regulated. Tens of thousands of genes have been sequenced for the model plant <em>Arabidopsis thaliana</em>, yet few have been functionally annotated and characterized. This ... -
Characterization of HSP47 Expression in <i>Xenopus Laevis</i> Cell Culture and Embryos
(University of Waterloo, 2005)The heat shock or stress response is a transient response to stressful stimuli that protects vital cellular proteins from damage and irreversible aggregation. Heat shock proteins (Hsps) are molecular chaperones that ... -
Characterization of iron- and zinc-containing alcohol dehydrogenases from anaerobic hyperthermophiles
(University of Waterloo, 2007-12-18)Hyperthermophiles are microorganisms that can grow at temperatures close to the boiling point of water or above. They are potential resources of thermostable enzymes including alcohol dehydrogenases (ADHs). Both Thermococcus ... -
Characterization of Mrc1 during Replication Initiation in Saccharomyces cerevisiae
(University of Waterloo, 2022-01-12)DNA replication is a highly specific process that is completed under the control of several factors, to ensure faithful duplication of the genome. Mrc1 (Claspin in mammalian cells) is a protein that is a part of a fork ... -
Characterization of novel FHA domain ligand binding motifs
(University of Waterloo, 2016-01-25)Rad53, a key checkpoint protein in Saccharomyces cerevisiae, has a central catalytic kinase domain as well as two forkhead-associated (FHA) domains, FHA1 and FHA2. Along with the extensively studied phosphothreonine binding ... -
Characterization of Novel Protease Flagellinolysin in Pseudoalteromonas tunicata
(University of Waterloo, 2024-01-03)Flagellinolysins are a family of novel enzymes that act both as structural flagellins and proteases. Although previously identified and shown to be both structurally integrated into filaments and proteolytically active, ... -
Characterization of Orc6 function following pre-replicative complex assembly in Saccharomyces cerevisiae
(University of Waterloo, 2008-10-22)Pre-replicative complex (pre-RC) components the origin recognition complex (ORC), Cdc6, and Cdt1, play key roles in the recruitment, and loading of the replicative helicase, the minichromosome maintenance complex (Mcm2-7), ... -
Characterization of p53 in rainbow trout cell lines
(University of Waterloo, 2010-09-27)The tumour suppressor protein p53 is a critical protein in the DNA damage checkpoint pathway. It acts as a transcription factor that is involved in initiating the apoptotic pathway; disruption of this pathway can lead to ... -
Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae.
(University of Waterloo, 2012-01-03)Initiation of DNA replication requires the action of the Dbf4/Cdc7 kinase complex (DDK) which is also a phosphorylation target of Rad53 kinase in the S-phase checkpoint. DDK is thought to trigger DNA replication by ... -
Characterization of the chloroplast protein import receptors in the single-cell C4 species Bienertia sinuspersici
(University of Waterloo, 2012-04-25)Bienertia sinuspersici is one of the three currently known land plants which perform C4 photosynthesis without the conventional dual-cell system, but rather through intracellular compartmentation of organelles and enzymes ... -
Characterization of the Downstream Effects of the Rainbow Trout (Oncorhynchus mykiss) Chemokine, CK-2 on Macrophages
(University of Waterloo, 2010-05-25)Chemokines are chemotactic cytokines that have the ability to attract leukocytes and guide them via a concentration gradient to sites of injury/infection. These small, basic proteins are secreted when induced and act on ... -
Characterization of the expression and function of <em>Rana catesbeiana</em> HSP30 and <em>Xenopus laevis</em> HSP27
(University of Waterloo, 2006)Exposure of cells to environmental or chemical stressors will initiate the heat shock response, which is mediated by heat shock proteins. Heat shock proteins are molecular chaperones which are classified by size into six ... -
Characterization of the FHA domain involved in Saccharomyces cerevisiae dNTP regulation.
(University of Waterloo, 2018-04-26)Over 2000 Forkhead-associated (FHA) domain-containing proteins exhibiting diverse functions, such as kinases, phosphatases, and transcription factors, have been identified to date in both eukaryotic and prokaryotic organisms. ... -
Characterization of the Mechanistic Roles of Rif1-Glc7 in DNA Double-Strand Break Repair Pathway Choice in Saccharomyces cerevisiae
(University of Waterloo, 2023-05-10)Rap-1 interacting factor 1 (Rif1) is a protein involved in telomere regulation, DNA replication and has more recently been found to mediate genomic stability by influencing the choice of DNA double-strand break (DSB) repair ... -
Characterization of the nodulation phenotype of E151, a pleiotropic pea (Pisum sativum L.) mutant.
(University of Waterloo, 2008-01-16)E151 (sym15) is characterized as a pleiotropic pea (Pisum sativum L.) mutant. It has been described as having short lateral roots, a short primary root, and a shorter epicotyl than that of the wild type Sparkle. Furthermore, ... -
Characterization of the pro-apoptotic function of eIF5A in human cancer cell lines
(University of Waterloo, 2007-09-18)Eukaryotic translation initiation factor 5A (eIF5A) is the only known protein containing the unique amino acid, hypusine. eIF5A is present in all eukaryotic cells and is highly conserved, but its function is not well ... -
Characterization of the role of Orc6 in the cell cycle of the budding yeast <em>Saccharomyces cerevisiae</em>
(University of Waterloo, 2006)The heterohexameric origin recognition complex (ORC) acts as a scaffold for the G1 phase assembly of pre-replicative complexes. Only the Orc1-5 subunits are required for origin binding in budding yeast, yet Orc6 is an ... -
Characterization of Two Novel Voltage-gated Calcium Channel Beta Subunits from Lymnaea stagnalis
(University of Waterloo, 2010-03-01)In excitable tissues, voltage-gated calcium channel activity is critical in the linkage of electrical stimuli to physiological responses, and so modulation of calcium channels therefore has significant implications. The ... -
The characterization of γH2AX and p53 as biomarkers of genotoxic stress in a rainbow trout (Oncorhynchus mykiss) brain cell line
(Elsevier, 2018-06-01)Rainbow trout cell cultures were exposed to three genotoxicants and examined for effects on γH2AX and p53 levels by western blotting and on cell viability using the indicator dyes Alamar Blue (AB) for energy metabolism and ... -
The characterization of γH2AX as a biomarker of genotoxicity in Oncorhynchus mykiss
(University of Waterloo, 2017-05-19)Environmental contamination due to by-products and waste from industrial activities is a growing universal concern, especially regarding the impact on water and the aquaculture industry. Conventional tests to diagnose ...